(1)英文名称:HMC3细胞,别称:CHME-3细胞;CHME3细胞;HMC-3细胞
(2)中文名称:人小胶质细胞
(3)HMC3细胞:HMC3细胞系通过人类胎儿脑源性原代小胶质细胞培养经SV40T依赖性永生建立。通常被应用于神经科学及神经炎症的研究。该永生化的小胶质细胞群能无限性生长,用于小胶质细胞的生化功能分析,它们也易于转染而通常用于转染基因的表达研究,具有原代小胶质细胞的特性。
(4)基因表达:Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).
(5)组织来源:人脑
(6)生长方式:贴壁生长
(7)细胞形态:巨噬细胞样
(8)HMC3细胞完全培养基:MEM+10%FBS+1%双抗
(9)培养条件:37℃,5%CO2
(10)规格:T25方瓶或1ml冻存管
(11)细胞数量:1×10^6
(12)运输方式:常温运输(T25方瓶)或干冰运输(冻存管)
(13)支原体检测:阴性
(14)STR profiling